Current Research at Michigan State University

Vitamin cycles such as the folic acid and homocysteine cycle are essential regulators of cell alignment, orientation, and development of the neural tube. If there is too little folic acid or too much homocysteine, then the neural tube will develop malformations. Neural tube defects still occur despite attempts to supplement mothers with folic acid, due to late supplementation or deficiencies in folic acid uptake. As a result, children are born with deformed spines, or pieces of their skull are missing. One of the most severe neural tube defects is spina bifida (SB). This neural tube defect affects the lumbar region of the back which innervates the bladder. Due to this disruption of innervation, when children are born, they do not have proper control of their lower bodies, especially their bladders. The improper neurological connection in SB patients leads to the development of neurogenic bladders. As soon as children affected by SB are born, their bladders are catheterized. The children cannot sense when they need to void their bladder and do not have proper control of it. The children do not have proper urological control because the extracellular matrix of the neurogenic bladder contains excess elastin and insufficient collagen. It has been shown that homocysteine can upregulate collagen production and degrade elastin networks.  Chelsie studies the effect of homocysteine and folic acid on the extracellular matrix of bladder smooth muscle cells. This is done by culturing rat bladder smooth muscle cells on a pre-stretched polydimethylsiloxane surface to model a SB neurogenic bladder. In addition, the growth rate of the smooth muscle cells and their alignment are evaluated on the pre-stretched surface. She hypothesizes that this model would allow her to investigate the effect of homocysteine and mechanical cue on the extracellular matrix of the bladder smooth muscle cells.

Past Research at Texas A&M University

This is past research I conducted at Texas A&M University. At the 2016 FIU McNair Conference I won 1st Place Oral Presentation in Engineering, Physics, Mathematics and Computer Science for my research in Raman Spectroscopy

Past Research at Florida International University Mass Dynamics Laboratory

Histology Research Assistant (Miami, FL January 2014 – April 2015)
• Experimented with different brain abnormalities regarding epilepsy to lessen the seizures in the brain
• Prepared slides of rat brain sections by making solutions and sectioned different parts of the brain
• Experimented with varying protocols of staining to analyze different cells in the brain to see how they react under different microscopy analysis such as fluorescent microscopy

Skills​

• Languages: MATLAB, Python, Latex, Mathematica
• Software: Microsoft Office, Adobe Suite, MiniTab, Igor Pro, InkScape, Gimp, Krita, Figma, TinkerCad
• Lab Skills: Circuit development, soldering, cell culture, Western Blot, Raman Spectroscopy, thermal imaging, primary cell isolation, histology, Confocal Microscopy, Plasma Etching, 3D printing